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PCR Detection Kit for Chlamydia caviae
PCR Detection Kit for Chlamydia caviae
豚鼠衣原体PCR检测试剂盒
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Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 


Chlamydia caviae, also known as Chlamydia caviae/Chlamydia caviae, is an obligate intracellular parasitic prokaryote, rod-shaped, Gram-negative, once classified as Chlamydia psittaci . The currently known host of Chlamydia guinea pigs is limited to guinea pigs. In recent years, there have also been reports of infecting humans. It is not yet certain whether it can be transmitted between humans. Guinea pigs are sometimes infected in a subclinical state and sometimes show symptoms. The known symptoms are limited to these types: mild eyelid redness, conjunctivitis with thin or purulent discharge, rhinitis, and genital tract infection, and also related to abortion. and lower respiratory tract infections reported. The infection is self-limiting and resolves within 3-4 weeks.

 

The detection of chlamydia in guinea pigs was initially carried out through Conjunctival cells are stained with Giemsa or Macchiavello to observe whether there are characteristic cytoplasmic endosomes, but the sensitivity is low. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. The sensitivity is much higher than that of staining. It has strong specificity and can determine the type of bacterial infection. The detection speed is fast and only takes two or three hours to complete, so it can be effectively Detection of Chlamydia in Guinea Pigs by Alternative Staining.

 

This kit targets the 16S-23S rRNA gene interval Sequence designed primers can specifically identify Chlamydia guinea pig. Verified by BLAST, there is no intersection with the genomes of other organisms.fork reaction. This kit detected 9 different types of chlamydia, 20 types of bacteria and 4 types of viruses that may cause similar symptoms, and found no non-specific signals; among 405 chlamydia-positive samples, only 1 guinea pig sample was found to be positive. This kit can be used for the detection and identification of Chlamydia in guinea pigs.

 

-20℃避光保存,2-8℃运输,有效期一年。避免反复冻融。

 

 

豚鼠衣原体(Chlamydia caviae),也称为豚鼠嗜衣原体/豚鼠亲衣原体(Chlamydophila caviae),是一种专性细胞内寄生的原核生物,呈杆状,革兰氏阴性,曾被归类为鹦鹉热衣原体(Chlamydia psittaci)。豚鼠衣原体目前已知的宿主限于豚鼠,近年也有感染人类的报道,尚不确定是否可以在人类之间传播。豚鼠感染后有时处于亚临床状态,有时表现出症状,已知的症状限于这几种类型:轻度眼皮红肿、带有稀薄或脓性分泌物的结膜炎、鼻炎以及生殖道感染,也有关于流产和下呼吸道感染的报道。感染带有自限性,可在3-4周内自愈。

对豚鼠衣原体的检测,最初是通过对结膜细胞进行Giemsa或Macchiavello染色,观察是否有特征性的细胞质内涵体,灵敏度低。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度远高于染色法,特异性强,可以确定感染的细菌种类,检测速度快,只需两三个小时即可完成,因此可以有效替代染色法检测豚鼠衣原体。

本试剂盒针对16S-23S rRNA基因区间序列设计引物,可以特异性识别豚鼠衣原体,经BLAST验证,与其他生物基因组没有交叉反应。本试剂盒检测了9种不同的衣原体,和可能引起类似症状的20种细菌和4种病毒,未发现非特异性信号;在405个衣原体阳性样本中,仅在1个豚鼠样本中发现阳性。本试剂盒可用于豚鼠衣原体的检测和鉴定。


 

 

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