Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Ureaplasma is classified as a mycoplasma and is one of the smallest free-living organisms. It has no cell wall and limited biosynthetic capacity. It needs to absorb cholesterol from the environment and hydrolyze urea to produce ATP for metabolism. It is generally believed that Ureaplasma is a commensal bacterium in the genitourinary tract of sexually active adults. The positive rate in women is about 40-80%. It is sometimes associated with some diseases, such as: urethritis, postpartum endometritis, chorionic villi Amnionitis, spontaneous abortion, premature birth, low birth weight, pneumonia, bacteremia, meningitis and chronic lung disease of premature infants, etc. It is unclear under what circumstances Ureaplasma causes disease, and people with intact humoral immune systems are generally unaffected.

To date, Ureaplasma parasitizes humans There are 14 known serotypes, which were originally collectively called Ureaplasma urealyticum . With the development of technology, after years of debate, these serotypes were classified into two species in 2002: 4 serotypes were assigned to Ureaplasma parvum Ureaplasma serotypes), the remaining 10 serotypes are still classified as Ureaplasma urealyticum . The genome size of Ureaplasma parvum ranges from 0.75–0.78 Mbp, and the genome size of the differentiated Ureaplasma urealyticum ranges from 0.84–0.95 Mbp. There is currently no consistent conclusion on the impact of different serotypes on human diseases.

The in vitro culture of Ureaplasma is very difficult, time-consuming and has low sensitivity. Typing relies on monoclonal or polyclonal antibodies against whole cells or purified antigens. Experimental methods include: growth inhibition experiment, metabolic inhibition experiment, immunofluorescence, immunoperoxidase, ELISA, western blot, etc. These methods are relatively time-consuming. It is time-consuming and labor-intensive, and the results are often difficult to repeat. There are many cross-reactions, and it is often difficult to distinguish between samples containing multiple serotypes. The PCR method used to detect Ureaplasma has higher sensitivity and better specificity. Affected by other microbial contamination, and the detection time is short, results can be obtained in just a few hours.

This kit selects urease unique to Ureaplasma as the target, and can specifically identify all fourteen serotypes of Ureaplasma parvum and Ureaplasma urealyticum , verified by BLAST, there is only a weak cross-reaction with Ureaplasma diversum and no cross-reactivity with other biological genomes. Ureaplasma diversum lives in the urogenital tract of cattle and only Just be careful to avoid contamination when preparing samples, and it will not affect the detection of this kit. 12 other mycoplasma species, 4 species of bacteria with urease, and human genome were detected, and there was no specific reaction. . Among 250 genitourinary tract samples, 194 Ureaplasma positives were detected, which was consistent with the results of the culture method. It can be seen that this kit can be used for the detection and identification of Ureaplasma.

This product is for scientific research only.

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

脲原体(Ureaplasma)分类上属于支原体，是最小的可自由生活生物之一。它没有细胞壁，生物合成能力有限，需从环境吸收胆固醇，水解尿素产生ATP以供代谢。一般认为脲原体是性活跃成年人的泌尿生殖道共生菌，在女性中阳性率约在40-80%，有时会与一些疾病相关联，如：尿道炎、产后子宫内膜炎、绒毛膜羊膜炎、自然流产、早产、低出生体重、肺炎、菌血症、脑脊膜炎和早产儿慢性肺病等。目前尚不清楚脲原体会在什么情况下引起疾病，体液免疫系统健全的人一般不会受其影响。

迄今为止，寄生于人类的脲原体已知有14个血清型，最初被统称为解脲脲原体（Ureaplasma urealyticum，也有译为溶脲脲原体）。随着技术的发展，经过多年的争论，在2002年将这些血清型分属于两个物种：4个血清型归属于微小脲原体（Ureaplasma parvum，也有译为细小脲原体）（含：UPA1, UPA3,UPA6, UPA14），其余10个血清型仍归属于解脲脲原体（含：UUR2, UUR4, UUR5, UUR7, UUR8, UUR9, UUR10, UUR11, UUR12, 和UUR13）。微小脲原体的基因组大小在0.75–0.78 Mbp之间，区分后的解脲脲原体的基因组大小在0.84–0.95 Mbp。不同血清型对人体疾病的影响目前尚没有一致结论。

脲原体的体外培养非常困难，耗时且灵敏度较低。对脲原体的分型是依靠针对全细胞或纯化抗原的单克隆或多克隆抗体，实验方法包括：生长抑制实验、代谢抑制实验、免疫荧光、免疫过氧化物酶、ELISA、免疫印迹等，这些方法都比较耗时费力，结果常常难以重复，有很多交叉反应，在含有多个血清型的样本上常难以区分。而PCR法用于检测脲原体，则有更高的灵敏度和更好的特异性，不受其他微生物污染的影响，且检测时间短，仅需几个小时即可获得结果。

本试剂盒选择脲原体特有的尿素酶（urease）作为靶点，可以特异性识别微小脲原体以及解脲脲原体全部十四种血清型，经BLAST验证，仅与差异脲原体（Ureaplasma diversum）有很弱的交叉反应，与其他生物基因组没有交叉反应。差异脲原体生活在牛的泌尿生殖道，只需在准备样品时注意避免污染即可，不会对本试剂盒的检测造成影响。检测12种其他支原体（共27个菌株）、4种带有尿素酶的细菌以及人类基因组，均无特异性反应。在250个泌尿生殖道样品中，检测出194个脲原体阳性，与培养法结果一致，可见本试剂盒可用于脲原体的检测和鉴定。

本产品仅供科学研究使用。

 

 

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