Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

Many mycoplasmas are harmful to animals, humans, and plants Pathogenic, therefore they are of great importance in medicine and phytopathology. Mycoplasmas tend to be host-specific, and ruminants, especially cattle, harbor many specific parasitic mycoplasmas. Mycoplasma bovigenitalium, also known as Mycoplasma bovigenitalium, is considered to be related to bovine reproductive system diseases and can cause bovine genital tract inflammation, epididymitis and infertility, ranging from mastitis to mastitis. Mycoplasma bovine genitalium can also be isolated from sick cattle with pneumonia, conjunctivitis and arthritis.


Microbiological culture methods and immunological techniques are usually used for the detection of Mycoplasma in the bovine genital tract and identification. However, the former technique is very time-consuming and the latter technique is not sensitive enough in some cases. Mycoplasma bovis is highly similar to Mycoplasma bovis in biochemical characteristics and culture methods. Although they can be distinguished by some serological techniques, cross-reactivity also exists. Using PCR technology to amplify the 16S rRNA gene in vitro can be used to identify and detect Mycoplasma in the bovine genital tract, with the advantages of high sensitivity and short detection time.


The bovine genital tract Mycoplasma PCR detection kit is species-specific. The primers used in the kit have been verified by BLAST to specifically target Mycoplasma bovine genital tract and do not cross-react with the genomes of other organisms. This kit was used to detect 25 species of mycoplasma, including 16 species of bovine parasitic Mycoplasma and 6 species of ovine parasitic Mycoplasma. Only Mycoplasma bovine genital tract can produce amplified bands. For the four types of human mycoplasmas that often cause cell contamination, this kit has no cross-reactivity, so it can avoid potential contamination from manual operations.


Isolation from calf nasal swabs or cow udder milk samples collected from farms The 95 mycoplasma-like colonies that came out were identified simultaneously using this kit and conventional identification techniques, and the test results were completely consistent. It can be seen that this kit can be used to identify and detect bovine genital tract mycoplasma.

This product is for scientific research only.

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

许多支原体对动物、人类和植物都是致病的，因此它们在医学和植物病理学中非常重要。支原体往往是宿主特异性的，反刍动物，尤其是牛，拥有许多特异性的寄生支原体。牛生殖道支原体(Mycoplasma Bovigenitalium)，或称为牛生殖支原体，被认为与牛生殖系统疾病有关，可引起牛生殖道炎、附睾炎和不育症，从患乳腺炎、肺炎、结膜炎和关节炎的病牛中也可分离出牛生殖支原体。

微生物培养法和免疫学技术通常用于牛生殖道支原体的检测和鉴定。 但是，前一种技术非常耗时，而后一种技术在某些情况下不够灵敏。牛生殖道支原体与牛支原体（Mycoplasma Bovis）在生化特征和培养方法上高度类似。虽然它们可以通过一些血清学技术加以区分，但也存在交叉反应。使用PCR技术体外扩增16S rRNA基因检测，可以用于鉴别和检测牛生殖道支原体，具有灵敏度高、检测时间短的优势。

牛生殖道支原体PCR检测试剂盒具有物种特异性。试剂盒所用的引物经BLAST验证为特异性靶向牛生殖道支原体，与其他生物的基因组不产生交叉反应。使用本试剂盒检测了25种支原体，包括16种牛寄生性支原体和6种羊寄生性支原体，仅有牛生殖道支原体可以产生扩增条带。对于经常造成细胞污染的4种人源支原体(口颊支原体Mycoplasma Buccale，口腔支原体Mycoplasma Orale，发酵支原体Mycoplasma Fermentans，人型支原体Mycoplasma  Hominis)，本试剂盒没有交叉反应，因此可以避免人工操作的潜在污染。

对从农场收集的小牛鼻拭子或奶牛乳房乳样品中分离出来的95个支原体样菌落，使用本试剂盒和常规鉴定技术同步进行鉴定，检测结果完全一致，可见本试剂盒可以用于鉴别和检测牛生殖道支原体。

 本产品仅供科学研究使用。 

 

 

 

 

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