Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit Features:
1. Specific detection of Mycoplasma mycoides without cross-reaction with other biological genomes.
2. High sensitivity, the detection limit is equivalent to 100fg/reaction.
3. Use hot-start DNA polymerase to inhibit non-specific amplification and reduce background fluorescence.
4, with positive control sample, which can be used to test the effectiveness of the kit.
5, with ROX reference dye to help correct sampling errors and differences between tubes.
6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.
Mycoplasma mycoides is a group of infectious mycoplasmas, including six members: Mmc, MmmLC, MmmSC, Mycoplasma leachii, Mcc and Mccp . These members cause a variety of diseases in ruminants ranging from mild to severe and fatal.
Mycoplasma mycoides filamentous subspecies small clone is a member of the Mycoplasma mycoides family. Because Mycoplasma mycoides subspecies large clone has been reclassified as Mycoplasma mycoides subspecies goat, therefore, Mycoplasma mycoides subsp. mycoides, referred to as Mmm) only MmmSC remains.
MmmSC was first discovered in 1898 and is transmitted by cattle The causative bacteria of CBPP manifests as serum fibrinization, interstitial pneumonia and pulmonary osteonecrosis. It is a major threat to the cattle industry. It can cause significant economic losses in endemic areas. Limited by the lack of small animal disease models, its pathogenic mechanism remains unclear, and polysaccharide antigens on its cell surface may be the causative factor. European strains have lower morbidity and fatality rates than African strains, which may be related to the release ofIt is less related to releasing hydrogen peroxide. In the early stages of the disease epidemic, acute and subacute onset are the main symptoms, and then the disease turns to chronic development.
Mmc can cause mastitis, joint disease in goats inflammation, lung disease and sepsis. MmmLC can cause pleuropneumonia, mastitis, arthritis and conjunctivitis in goats. It is very similar to MmmSC and was once mistaken for the same species. Analysis of the 16S rRNA sequence showed that MmmLC was 99.9% similar to Mmc, so they were classified as the same species. MmmLC is very widely distributed and can be found in small ruminants on all continents. It can be found in areas where infectious agalactiasis and goat pleuropneumonia are endemic. Due to the lack of diagnostic methods, its prevalence may be underestimated.
Serological methods are often used when detecting Mycoplasma mycoides Cross-reactivity and low sensitivity, and in vitro culture methods are more time-consuming. PCR is a highly sensitive and specific detection method that only takes a few hours to obtain results, which can effectively make up for the shortcomings of the above two methods. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.
This kit combines several Mycoplasma mycoplasma Compare the genomes of family members and select a common region as a target to specifically identify Mycoplasma mycoides, including: MmmSC, MmmLC and Mmc. It has been verified by BLAST that there is no cross-reaction with the genomes of other organisms. This kit detects a total of 68 strains of six members of the Mycoplasma mycoides family. Only 37 strains of Mycoplasma mycoides produced specific signals. The remaining members, as well as 7 other mycoplasma and 21 strains with similar distribution ranges, Bacteria produced no specific signal.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性检测丝状支原体（MmmSC、MmmLC和Mmc），与其他生物基因组没有交叉反应。
2， 灵敏度高，检测极限相当于100fg/反应。
3， 采用热启动DNA聚合酶，可抑制非特异性扩增，降低背景荧光。
4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。
5， 带有ROX参比染料，帮助校正加样误差和管间差异。
6， 带有UDG酶和dUTP，可降低残留DNA的污染。
丝状支原体族（Mycoplasma Mycoides cluster）是一组具有传染性的支原体，包括六个成员： Mmc（Mycoplasma mycoides subsp. Capri.）、MmmLC (Mycoplasma mycoides subsp. mycoides Large Colony，现已被重新归类至Mmc)、MmmSC (Mycoplasma mycoides subsp. mycoides Small Colony) 、Mycoplasma leachii (Mycoplasma sp. bovine group 7, Mbg7)、Mcc (Mycoplasma capricolum subsp. capricolum)和Mccp（Mycoplasma capricolum subsp. capripneumoniae)。这些成员在反刍动物中引起从较温和到严重致死的多种疾病。
丝状支原体丝状亚种小克隆（Mycoplasma mycoides subsp. mycoides SC，简称MmmSC）是丝状支原体族的一员。由于丝状支原体丝状亚种大克隆（Mycoplasma mycoides subsp. mycoides LC，简称MmmLC）已被重新归类为丝状支原体山羊亚种（Mycoplasma mycoides subsp. capricolum）,因此，丝状支原体丝状亚种（Mycoplasma mycoides subsp. mycoides，简称Mmm）仅剩MmmSC。
MmmSC于1898年首次发现，是牛传染性胸膜肺炎（CBPP，一种严重的呼吸道传染病，又称牛肺疫）的致病菌，表现为血清纤维蛋白化、间质性肺炎和肺部骨坏死，是养牛业的主要威胁，在流行区域可造成很大的经济损失。受限于缺乏小型动物的疾病模型，其致病机制仍不清楚，其细胞表面的多糖抗原可能是致病因素。欧洲菌株在发病率和致死率上低于非洲菌株，可能与其释放过氧化氢较少有关。在疾病流行的初期，以急性和亚急性发病为主，随后转向慢性发展。
Mmc可以在山羊中引起乳腺炎、关节炎、肺部疾病和败血症。MmmLC 可以在山羊中引起胸膜肺炎、乳腺炎、关节炎和眼结膜炎，与MmmSC相似度很高，曾被误认为是同一个物种。对16S rRNA序列的分析表明，MmmLC与Mmc有99.9%的相似度，因此将二者归为同一物种。MmmLC分布非常广泛，在各大陆的小型反刍动物中都有存在，在传染性无乳症和山羊胸膜肺炎流行地区都可发现其踪影，由于缺乏诊断方法，其流行程度可能被低估。
检测丝状支原体时血清学方法常出现交叉反应，且灵敏度较低，而体外培养法又较为耗时。PCR是一种高灵敏度和高特异性的检测方法，仅需几个小时即可获得结果，可以有效弥补上述两种方法的不足。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。
本试剂盒通过将几种丝状支原体族成员的基因组进行比较，选取一段共同区域作为靶点特异性识别丝状支原体，包括：MmmSC、MmmLC和Mmc，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了丝状支原体族六个成员共68个菌株，只有丝状支原体（MmmSC、MmmLC和Mmc）37个菌株产生特异性信号，其余成员，以及分布范围相近的7种其他支原体和21种细菌，均未产生特异性信号。
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