Applicable samples

Animal tissue

Product introduction

High-fat sample protein extraction reagent The cartridge is suitable for extracting total protein from various cells and solid tissues with high fat content, such as adipocytes, fatty liver cells, brain, fat, fatty liver, connective tissue, thymus tissue and other animal tissues. The extraction process is simple and convenient and can be completed within 1 hour.

The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins.

The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gel shift gel retardation experiment, enzyme activity determination, etc.

The protein extracted by this kit is an active protein with a natural protein conformation and has a wide range of downstream applications. The ability of the extract to lyse cells is relatively mild, and the lysis time needs to be optimized based on the actual sample conditions.

This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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