Applicable samples

Animal whole blood

Product introduction

Extraction of total protein from whole blood The kit is suitable for extracting total protein from anticoagulated/non-anticoagulated whole blood samples of various animals.

The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes.

The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins.

The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gel shift gel retardation experiment, enzyme activity determination, etc.

All components of this kit do not contain detergent components. The components of the final protein sample have no detergent effect on downstream applications such as NI column purification, molecular sieves, ion exchange, and affinity purification. .

The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, Triton X100, chaps and other components that may affect the mass spectrometry experiment. The final protein sample After dialysis or desalting, it will not contain detergents, high-concentration salts, etc., and can basically meet any downstream proteomics-related experimental research.

The protein extracted by this kit is an active protein with a natural protein conformation and has a wide range of downstream applications. The ability of the extract to lyse cells is relatively mild, and the lysis time needs to be optimized based on the actual sample conditions.

This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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