The Insect Nuclear Protein Extraction Kit provides a complete set of reagents suitable for extracting nuclear and cytoplasmic proteins from various insect cells and various insect solid tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nuclear proteins and cytoplasmic proteins not only have high purity and maintain natural activity, but also have very little cross-contamination. This kit contains a unique formula that can effectively dissolve nuclear membrane components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for Western Blotting, transcription activity analysis, Gelshift gel retardation experiment, co-immunoprecipitation, enzyme activity determination and other protein research. If your downstream applications are experiments such as metal chelation and chromatography that cannot contain EDTA reagents, you may want to look at EDTA-free protease inhibitor cocktails. The proteins extracted by this kit are active proteins with natural protein conformation.

 

Applicable samples: insects

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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