Mycoprotein extraction kit is suitable for extracting total protein from various large fungi (mushrooms) samples. The components of the kit are optimized for thick cell wall samples, and the extraction process is simple and convenient, and can be completed within 1 hour. The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: fungi

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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