Transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of diseases. The preparation of transmembrane protein samples requires full consideration of downstream applications such as gel analysis and mass spectrometry analysis. Therefore, the preparation of transmembrane protein samples has become an insurmountable challenge. The traditional method of preparing transmembrane protein samples is to solubilize them with detergents and surfactants. Detergent treatment can cause transmembrane proteins to lose their native structure, thus hampering functional studies of transmembrane proteins. Plant Transmembrane Protein Extraction Kit is a high-yield transmembrane protein extraction kit based on chemical methods. The plant transmembrane protein extraction kit can extract total transmembrane proteins from various plants and can be used for the preparation of crude purified proteins and the preparation of transmembrane proteins. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protein degradation by proteases and ensures the extraction of high-quality proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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