Transmembrane proteins undertake various biological functions. The preparation of membrane protein samples needs to fully consider the downstream applications such as gel analysis and mass spectrometry analysis. Therefore, the preparation of membrane protein samples has become an insurmountable challenge. The Yeast Membrane Protein/Cytoplasmic Protein Extraction Kit is an efficient and high-yield membrane protein extraction kit. The Yeast Membrane Protein/Cytoplasmic Protein Extraction Kit can extract membrane proteins/cytosolic proteins from various yeast samples and can be used for purification. Crude protein preparation and membrane protein preparation. The extraction process is simple and convenient. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: yeast

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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