mitochondria is an important organelle that produces energy in eukaryotic cells. The energy substances in the cell—fat, sugar, and some amino acids—are ultimately oxidized here and generate ATP through coupled phosphorylation to supply cellular physiological activities. needs. Transmembrane proteins undertake various biological functions and play an important role in various biological development processes. The preparation of membrane protein samples needs to fully consider the downstream applications such as gel analysis and mass spectrometry analysis, so the preparation of membrane protein samples has become an insurmountable challenge. The Plant Mitochondrial Membrane Protein Extraction Kit uses a simple and fast method to extract mitochondrial membrane proteins from various plant samples. The extraction process is simple and convenient, and high-quality mitochondrial membrane proteins can be extracted within one hour. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protein degradation by proteases and ensures the extraction of high-quality proteins. This kit contains a unique formula that effectively dissolves membrane components. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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