The Mitochondrial Protein/Cytoplasmic Protein Extraction Kit provides a complete set of reagents, suitable for extracting from various primary or passaged cells and various solid tissues, such as brain, spinal cord, neural nodes or fibers, fat, liver, digestive tract, kidney Extract mitochondrial proteins and cytoplasmic proteins from mammalian tissues such as heart, muscle, blood vessels, connective tissue, etc. The extraction process is simple and convenient. The prepared mitochondrial protein not only has high purity and maintains natural activity, but also has very little cross-contamination. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: animal tissue

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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