The Cytoplasmic Protein Extraction Kit provides a complete set of reagents suitable for the preparation of cytoplasmic proteins from cells and animal tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared cytoplasmic protein not only has high purity and maintains natural activity, but also has very little cross-contamination. All components of this kit do not contain detergent components, and the components of the final protein sample have no detergent impact on downstream applications such as NI column purification, molecular sieves, ion exchange, and affinity purification. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with a natural protein conformation and has a wide range of downstream applications. The ability of the extract to lyse cells is relatively mild, and the lysis time needs to be optimized based on the actual sample conditions. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: animal cells/tissues

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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