The Plant Nuclear Protein Extraction Kit (without detergent components) provides a complete set of reagents and is suitable for extracting nuclear proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nuclear protein not only has high purity and maintains natural activity, but also has very little cross-contamination. All components of this kit do not contain detergent components, and the components of the final protein sample have no significant impact on downstream applications such as NI column purification, molecular sieves, ion exchange, and affinity purification. This kit contains a unique formula that can effectively dissolve plant core components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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