Yeast protein extraction kit (suitable for proteomics experiments) is suitable for efficient and gentle extraction of total soluble proteins from various yeast samples. The extraction process is simple and convenient, avoiding the damage to yeast cells caused by grinding, ultrasonic or pressing methods with poor repeatability, and avoiding the damage to the target protein caused by oxidation and increased heat caused by intense mechanical processing. The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment. The final protein sample is dialyzed or desalted. It will not contain the effects of detergents, high-concentration salts, etc., and can basically meet any downstream proteomics-related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid mass spectrum peak drift caused by AEBSF. Therefore, protein samples extracted with this product can be used for mass spectrometry (MS) detection and analysis, proteomics, etc. Related research. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography. The protein extracted by this kit can also be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, and enzyme activity determination.

 

Applicable samples: yeast

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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