TEL: +86 571 56623320 EMAIL: SALES@SUNLONGBIOTECH.COM
Drug Names
Generic Name: Chicken Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit
Purpose
Our Chicken Tartrate Resistant Acid Phosphatase(TRAP) ELISA kit is to assay TRAP levels in Chicken serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TRAP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for TRAP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TRAP and HRP conjugated TRAP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TRAP. You can calculate the concentration of TRAP in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode