TEL: +86 571 56623320    EMAIL: [email protected]

One Step ELISA Kit For Mouse Interleukin 6 (IL6)
One Step ELISA Kit For Mouse Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Total
(Vip priceV)
Regular members: $524.0
  • Save more [Favourable] 45% Discount
  • NO.:One-EK001M
    Species:Mouse
    Assay range:7.81 pg/mL – 500 pg/mL
    Sensitivity:4.92 pg/mL
    Sample Volume:Serum, plasma:10 μL
    Assay type:One step sandwich elisa
    Validity:Two Years
    Assay Time:1.5 hours
    NCBI_Gene:3569
    UniProtKB:P05231
  • Goods click count:631
  • Product Spec:
  • Quantity: - +
  • Limit points for buying:0 Points
  • Manual References (0)
  • Add to cart Inquiry Add to favorite
View History [Clear]

Details

SAMPLE TYPE
Cell culture supernates, serum and plasma
SAMPLE VOLUME Serum or plasma: 10μL;  cell culture supernatant: 50μL
SENSITIVITY 4.92 pg/mL
RANGE 7.81 pg/mL – 500 pg/mL
ASSAY TIME 1.5 h
RECOVERY 89% – 112%
AVERAGE RECOVERY 1.02
INTRA PRECISION 2.3% – 3.0%
INTER-PRECISION 2.1% – 5.3%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Mouse IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

References (0)

No References

Bought notes(bought amounts latest0)

No one bought this product
Total 0 records, divided into1 pages First Prev Next Last

User Comment(Total0User Comment Num)

  • No comment
Total 0 records, divided into1 pages First Prev Next Last
Username: Anonymous user
E-mail:
Rank:
Content:
Verification code: captcha

Call us

+86 571 56623320

Address

Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China

Join Us with

Leave a message
* To protect against spam, please pass the CAPTCHA test below.