SAMPLE TYPE	Cell culture supernates, serum and plasma
SAMPLE VOLUME	Serum or plasma: 10μL;  cell culture supernatant: 50μL
SENSITIVITY	4.92 pg/mL
RANGE	7.81 pg/mL – 500 pg/mL
ASSAY TIME	1.5 h
RECOVERY	89% – 112%
AVERAGE RECOVERY	1.02
INTRA PRECISION	2.3% – 3.0%
INTER-PRECISION	2.1% – 5.3%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Mouse IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

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