Drug Names

Generic Name: Rat Collagen type Ⅱ (Col Ⅱ-Ab) Antibody ELISA kit

Purpose

Our Rat Collagen type Ⅱ (Col Ⅱ-Ab) Antibody ELISA kit is to assay Col Ⅱ-Ab levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This Antibody ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Col Ⅱ-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Col Ⅱ-Ab is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Col Ⅱ-Ab and HRP conjugated Col Ⅱ-Ab antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Col Ⅱ-Ab. You can calculate the concentration of Col Ⅱ-Ab in the samples by comparing the OD of the samples to the standard curve.

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