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Drug Names
Generic Name: Human Anti-Complement C3 Convertase Antibody (C3c-Ab) ELISA Kit
Purpose
Our Human Anti-Complement C3 Convertase Antibody (C3c-Ab) ELISA kit is to assay C3c-Ab levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to C3c-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for C3c is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain C3c-Ab and HRP conjugated C3c antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C3c-Ab. You can calculate the concentration of C3c-Ab in the samples by comparing the OD of the samples to the standard curve.
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