Summary:

 

Purpose

 

Our Quick Step Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT is to assay VEGF levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to VEGF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for VEGF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain VEGF and HRP conjugated VEGF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VEGF. You can calculate the concentration of VEGF in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0558Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.046	0.0435
		0.041
S1	24pg/ml	1.989	1.9925
		1.996
S2	16pg/ml	1.331	1.3420
		1.353
S3	8pg/ml	0.694	0.7025
		0.711
S4	4pg/ml	0.391	0.3865
		0.382
S5	2pg/ml	0.194	0.1975
		0.201

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