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Quick Step Mouse Methemoglobin ,MHB ELISA Kit
Quick Step Mouse Methemoglobin ,MHB ELISA Kit
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  • NO.:QS0376Mo
    Sensitivity:0.1 ng/ml
    Assay range:0.8 ng/ml -50 ng/ml
    Standard:72 ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

 

Summary:

 

Purpose

 

Our Quick Step Mouse Methemoglobin,MHB ELISA Kit is to assay MHB levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MHB. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MHB is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MHB and HRP conjugated MHB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MHB. You can calculate the concentration of MHB in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0376Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0ng/ml

0.046

0.0435

0.041

S1

24ng/ml

1.989

1.9925

1.996

S2

16ng/ml

1.331

1.3420

1.353

S3

8ng/ml

0.694

0.7025

0.711

S4

4ng/ml

0.391

0.3865

0.382

S5

2ng/ml

0.194

0.1975

0.201

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