Summary:

 

Purpose

 

Our Quick Step Human Phosphodiesterase 9A (PDE9A)elisa Kit is to assay PDE9A levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PDE9A. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PDE9A is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PDE9A and HRP conjugated PDE9A antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PDE9A. You can calculate the concentration of PDE9A in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3752Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/L	0.046	0.0435
		0.041
S1	24nmol/L	1.989	1.9925
		1.996
S2	16nmol/L	1.331	1.3420
		1.353
S3	8nmol/L	0.694	0.7025
		0.711
S4	4nmol/L	0.391	0.3865
		0.382
S5	2nmol/L	0.194	0.1975
		0.201

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