Summary:

 

Purpose

 

Our Quick Step Human Complement 1 (C1) ELISA Kit is to assay C1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to C1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for C1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain C1 and HRP conjugated C1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C1. You can calculate the concentration of C1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3455Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μg/ml	0.044	0.0480
		0.052
S1	60μg/ml	1.999	1.9865
		1.974
S2	40μg/ml	1.302	1.3115
		1.321
S3	20μg/ml	0.687	0.6900
		0.693
S4	10μg/ml	0.392	0.3855
		0.379
S5	5μg/ml	0.202	0.2030
		0.204

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