Summary:

 

Purpose

 

Our Quick Step Human 2,3 Bisphosphoglycerate (2,3-BPG)elisa Kit is to assay 2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 2 and HRP conjugated 2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 2. You can calculate the concentration of 2 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2702Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/ml	0.044	0.0480
		0.052
S1	60U/ml	1.999	1.9865
		1.974
S2	40U/ml	1.302	1.3115
		1.321
S3	20U/ml	0.687	0.6900
		0.693
S4	10U/ml	0.392	0.3855
		0.379
S5	5U/ml	0.202	0.2030
		0.204

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