Summary:

 

Purpose

 

Our Quick Step Human DNA topoisomerase 2-alpha (TOP2A)elisa Kit is to assay TOP2A levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TOP2A. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TOP2A is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TOP2A and HRP conjugated TOP2A antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TOP2A. You can calculate the concentration of TOP2A in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3236Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.044	0.0480
		0.052
S1	60pg/ml	1.999	1.9865
		1.974
S2	40pg/ml	1.302	1.3115
		1.321
S3	20pg/ml	0.687	0.6900
		0.693
S4	10pg/ml	0.392	0.3855
		0.379
S5	5pg/ml	0.202	0.2030
		0.204

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