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Summary:
Purpose
Our Quick Step Mouse Interleukin 1β(IL-1β)ELISA Kit is to assay IL- 1β levels in Mouse serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL- 1β. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL- 1β is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL- 1β and HRP conjugated IL- 1β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL- 1β. You can calculate the concentration of IL- 1β in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS0835Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0pg/ml
0.043
0.0475
0.052
S1
72pg/ml
1.988
1.9805
1.973
S2
48pg/ml
1.302
1.3095
1.317
S3
24pg/ml
0.692
0.7035
0.715
S4
12pg/ml
0.377
0.3855
0.394
S5
6pg/ml
0.202
0.1980
0.194
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