Summary:

 

Purpose

 

Our Quick Step Human anti-mutated citrullinated vimentin antibody, MCV ELISA Kit is to assay MCV levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to MCV. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for MCV is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MCV and HRP conjugated MCV antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MCV. You can calculate the concentration of MCV in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0214Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/ml	0.043	0.0475
		0.052
S1	72U/ml	1.988	1.9805
		1.973
S2	48U/ml	1.302	1.3095
		1.317
S3	24U/ml	0.692	0.7035
		0.715
S4	12U/ml	0.377	0.3855
		0.394
S5	6U/ml	0.202	0.1980
		0.194

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