TEL: +86 571 56623320 EMAIL: [email protected]
Summary:
Purpose
Our Quick Step Human hepatitis B virus surface IgG, HBs-IgG ELISA Kit is to assay HBs-IgG levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to HBs-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for HBs-IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HBs-IgG and HRP conjugated HBs-IgG antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HBs-IgG. You can calculate the concentration of HBs-IgG in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS3574Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0IU/L
0.043
0.0475
0.052
S1
72IU/L
1.988
1.9805
1.973
S2
48IU/L
1.302
1.3095
1.317
S3
24IU/L
0.692
0.7035
0.715
S4
12IU/L
0.377
0.3855
0.394
S5
6IU/L
0.202
0.1980
0.194
Scan Wechat Qrcode