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Quick Step Human beta Carotene (BCT)ELISA kit
Quick Step Human beta Carotene (BCT)ELISA kit
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  • NO.:QS2989Hu
    Sensitivity:10pg/mL
    Assay range:50pg/mL -1500pg/mL
    Standard:1800 pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

 

Summary:

 

Purpose

 

Our Quick Step Human beta Carotene (BCT)elisa Kit is to assay BCT levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BCT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for BCT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BCT and HRP conjugated BCT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BCT. You can calculate the concentration of BCT in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2989Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pg/ml

0.035

0.0385

0.042

S1

1200pg/ml

1.973

1.9790

1.985

S2

800pg/ml

1.327

1.3330

1.339

S3

400pg/ml

0.705

0.7105

0.716

S4

200pg/ml

0.393

0.3885

0.384

S5

100pg/ml

0.186

0.1895

0.193 

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