Summary:

 

Purpose

 

Our Quick Step Mouse Insulin-like growth factor binding protein 6,IGFBP-6 ELISA kit is to assay IGFBP-6 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGFBP-6. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IGFBP-6 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IGFBP-6 and HRP conjugated IGFBP-6 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IGFBP-6. You can calculate the concentration of IGFBP-6 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0298Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.04	0.04
		0.04
S1	1500pg/ml	1.91	1.91
		1.91
S2	1000pg/ml	1.37	1.37
		1.37
S3	500pg/ml	0.72	0.71
		0.71
S4	250pg/ml	0.41	0.40
		0.39
S5	125pg/ml	0.18	0.19
		0.20

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