Summary:

 

Purpose

 

Our Quick Step Human Anti-Deamidated Gliadin Peptide IgA,DGP-IgA ELISA Kit is to assay DGP IgA  levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to DGP IgA . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for DGP IgA  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain DGP IgA  and HRP conjugated DGP IgA  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DGP IgA . You can calculate the concentration of DGP IgA  in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2713Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Blank Well	0U/ml	0.049	0.0440
		0.039
S1	1800U/ml	1.956	1.9530
		1.950
S2	1200U/ml	1.389	1.3910
		1.393
S3	600U/ml	0.709	0.7135
		0.718
S4	300U/ml	0.390	0.3875
		0.385
S5	150U/ml	0.195	0.2000
		0.205

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