Summary:

 

Purpose

 

Our Quick Step Mouse Collagen Type II,Col II ELISA Kit is to assay Col II levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Col II. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Col II is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Col II and HRP conjugated Col II antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Col II. You can calculate the concentration of Col II in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0142Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.037	0.0400
		0.043
S1	3000pg/ml	1.994	1.9910
		1.988
S2	2000pg/ml	1.314	1.3110
		1.308
S3	1000pg/ml	0.714	0.7215
		0.729
S4	500pg/ml	0.398	0.3925
		0.387
S5	250pg/ml	0.200	0.1950
		0.190

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