Summary:

 

Purpose

 

Our Quick Step Porcine Amelogenin (AMEL)elisa Kit is to assay AMEL levels in Porcine serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AMEL. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for AMEL is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AMEL and HRP conjugated AMEL antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AMEL. You can calculate the concentration of AMEL in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0205Po is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.037	0.0400
		0.043
S1	3000pg/ml	1.994	1.9910
		1.988
S2	2000pg/ml	1.314	1.3110
		1.308
S3	1000pg/ml	0.714	0.7215
		0.729
S4	500pg/ml	0.398	0.3925
		0.387
S5	250pg/ml	0.200	0.1950
		0.190

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