Summary:

 

Purpose

 

Our Quick Step human Prostaglandin F,PG-F ELISA Kit is to assay PG-F levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PG-F. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PG-F is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PG-F and HRP conjugated PG-F antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PG-F. You can calculate the concentration of PG-F in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1464Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.037	0.0400
		0.043
S1	3000ng/L	1.994	1.9910
		1.988
S2	2000ng/L	1.314	1.3110
		1.308
S3	1000ng/L	0.714	0.7215
		0.729
S4	500ng/L	0.398	0.3925
		0.387
S5	250ng/L	0.200	0.1950
		0.190

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