Summary:

 

Purpose

 

Our Quick Step Mouse alpha-L-fucosidase,AFU ELISA Kit is to assay AFU levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AFU. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for AFU is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AFU and HRP conjugated AFU antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AFU. You can calculate the concentration of AFU in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0037Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
		0.040
S1	3600pg/ml	1.912	1.9075
		1.903
S2	2400pg/ml	1.388	1.3840
		1.380
S3	1200pg/ml	0.708	0.7100
		0.712
S4	600pg/ml	0.403	0.4000
		0.397
S5	300pg/ml	0.189	0.1960
		0.203

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