Summary:

 

Purpose

 

Our Quick Step Bovine Blood urea nitrogen (BUN)elisa Kit is to assay BUN levels in Bovine serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BUN. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for BUN is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BUN and HRP conjugated BUN antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BUN. You can calculate the concentration of BUN in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0086Bo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/ml	0.052	0.0460
		0.040
S1	3600nmol/ml	1.912	1.9075
		1.903
S2	2400nmol/ml	1.388	1.3840
		1.380
S3	1200nmol/ml	0.708	0.7100
		0.712
S4	600nmol/ml	0.403	0.4000
		0.397
S5	300nmol/ml	0.189	0.1960
		0.203

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