Summary:

 

Purpose

 

Our Quick Step Bovine Pepsin (PP)elisa Kit is to assay PP levels in Bovine serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PP and HRP conjugated PP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PP. You can calculate the concentration of PP in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0225Bo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
		0.040
S1	3600pg/ml	1.912	1.9075
		1.903
S2	2400pg/ml	1.388	1.3840
		1.380
S3	1200pg/ml	0.708	0.7100
		0.712
S4	600pg/ml	0.403	0.4000
		0.397
S5	300pg/ml	0.189	0.1960
		0.203

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