Summary:

 

Purpose

 

Our Quick Step Human beta2-Glycoprotein 1 Antibody IgM,beta2-GP1 Ab IgM ELISA Kit is to assay beta2-GP1Ab IgM levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antigen specific to beta2-GP1Ab IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antigen. Then a Horseradish Peroxidase (HRP)- conjugated Antigen specific for beta2-GP1Ab IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain beta2-GP1Ab IgM and HRP conjugated beta2-GP1Ab IgM Antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of beta2-GP1Ab IgM. You can calculate the concentration of beta2-GP1Ab IgM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1877Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μg/L	0.059	0.0560
		0.053
S1	360μg/L	1.975	1.9695
		1.964
S2	240μg/L	1.388	1.3800
		1.372
S3	120μg/L	0.705	0.6995
		0.694
S4	60μg/L	0.383	0.3800
		0.377
S5	30μg/L	0.197	0.2010
		0.205

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