Summary:

 

Purpose

 

Our Quick Step Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit is to assay BMP-4 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to BMP-4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for BMP-4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BMP-4 and HRP conjugated BMP-4 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BMP-4. You can calculate the concentration of BMP-4 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0096Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.059	0.0560
		0.053
S1	360ng/ml	1.975	1.9695
		1.964
S2	240ng/ml	1.388	1.3800
		1.372
S3	120ng/ml	0.705	0.6995
		0.694
S4	60ng/ml	0.383	0.3800
		0.377
S5	30ng/ml	0.197	0.2010
		0.205

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