Summary:

 

Purpose

 

Our Quick Step Sheep neutrophil elastase (NE)elisa Kit is to assay NE levels in Sheep serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to NE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for NE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NE and HRP conjugated NE Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NE. You can calculate the concentration of NE in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS00051Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.059	0.0560
		0.053
S1	360pg/ml	1.975	1.9695
		1.964
S2	240pg/ml	1.388	1.3800
		1.372
S3	120pg/ml	0.705	0.6995
		0.694
S4	60pg/ml	0.383	0.3800
		0.377
S5	30pg/ml	0.197	0.2010
		0.205

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