Summary:

 

Purpose

 

Our Quick Step Human Antithrombin IIi (AT III)elisa Kit is to assay AT III levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antigen specific to AT III. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antigen. Then a Horseradish Peroxidase (HRP)- conjugated Antigen specific for AT III is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AT III and HRP conjugated AT III Antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AT III. You can calculate the concentration of AT III in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3113Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.052	0.0530
		0.054
S1	480ng/ml	1.969	1.9715
		1.974
S2	320ng/ml	1.272	1.2705
		1.269
S3	160ng/ml	0.785	0.7885
		0.792
S4	80ng/ml	0.474	0.4785
		0.483
S5	40ng/ml	0.215	0.2190
		0.223

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