Summary:

 

Purpose

 

Our Quick Step Mouse p53/tumor protein,p53/TP53 ELISA Kit is to assay p53/TP53 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to p53/TP53. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for p53/TP53 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain p53/TP53 and HRP conjugated p53/TP53 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of p53/TP53. You can calculate the concentration of p53/TP53 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0427Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.047	0.0400
		0.033
S1	720pg/ml	1.958	1.9635
		1.969
S2	480pg/ml	1.393	1.3900
		1.387
S3	240pg/ml	0.715	0.7225
		0.730
S4	120pg/ml	0.375	0.3815
		0.388
S5	60pg/ml	0.196	0.1985
		0.201

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