Summary:

 

Purpose

 

Our Quick Step Sheep Total Protein (TP)ELISA Kit is to assay TP levels in Sheep serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to TP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for TP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TP and HRP conjugated TP Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TP. You can calculate the concentration of TP in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS00085Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μg/ml	0.047	0.0400
		0.033
S1	720μg/ml	1.958	1.9635
		1.969
S2	480μg/ml	1.393	1.3900
		1.387
S3	240μg/ml	0.715	0.7225
		0.730
S4	120μg/ml	0.375	0.3815
		0.388
S5	60μg/ml	0.196	0.1985
		0.201

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