Summary:

 

Purpose

 

Our Quick Step Human anti-cardiolipin antibody Igg (ACA-IgG)ELISA kit is to assay ACA-IgG  levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS2514Hu specific to ACA-IgG . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS2514Hu. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS2514Hu specific for ACA-IgG  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ACA-IgG  and HRP conjugated ACA-IgG  抗体QS2514Hu will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ACA-IgG . You can calculate the concentration of ACA-IgG  in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2514Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0单位	0.042	0.0485
		0.055
S1	120单位	1.998	1.9855
		1.973
S2	80单位	1.318	1.3240
		1.330
S3	40单位	0.688	0.6915
		0.695
S4	20单位	0.393	0.3875
		0.382
S5	10单位	0.201	0.1950
		0.189

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