Summary:

 

Purpose

 

Our Quick Step Mouse 5-Hydroxytryptamine,5HT ELISA Kit is to assay 5HT  levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5HT . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5HT  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 5HT  and HRP conjugated 5HT  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5HT . You can calculate the concentration of 5HT  in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0009Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0单位	0.042	0.0485
		0.055
S1	120单位	1.998	1.9855
		1.973
S2	80单位	1.318	1.3240
		1.330
S3	40单位	0.688	0.6915
		0.695
S4	20单位	0.393	0.3875
		0.382
S5	10单位	0.201	0.1950
		0.189

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