Summary:

 

Purpose

 

Our Quick Step Mouse Gelsolin ELISA Kit is to assay Gelsolin levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to Gelsolin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for Gelsolin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Gelsolin and HRP conjugated Gelsolin Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Gelsolin. You can calculate the concentration of Gelsolin in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0233Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.033	0.0360
		0.039
S1	900pg/ml	1.985	1.9810
		1.977
S2	600pg/ml	1.363	1.3695
		1.376
S3	300pg/ml	0.752	0.7480
		0.744
S4	150pg/ml	0.384	0.3805
		0.377
S5	75pg/ml	0.169	0.1780
		0.187

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