Summary:

 

Purpose

 

Our Quick Step Mouse monoamine oxidase,MAO ELISA Kit is to assay MAO levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to MAO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for MAO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MAO and HRP conjugated MAO Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MAO. You can calculate the concentration of MAO in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0380Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0mU/ml	0.033	0.0360
		0.039
S1	900mU/ml	1.985	1.9810
		1.977
S2	600mU/ml	1.363	1.3695
		1.376
S3	300mU/ml	0.752	0.7480
		0.744
S4	150mU/ml	0.384	0.3805
		0.377
S5	75mU/ml	0.169	0.1780
		0.187

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