Summary:

 

Purpose

 

Our Quick Step Mouse 8-Isoprostane (8-ISO)elisa Kit is to assay 8-ISO levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to 8-ISO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for 8-ISO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 8-ISO and HRP conjugated 8-ISO Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 8-ISO. You can calculate the concentration of 8-ISO in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0780Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.033	0.0360
		0.039
S1	900pg/ml	1.985	1.9810
		1.977
S2	600pg/ml	1.363	1.3695
		1.376
S3	300pg/ml	0.752	0.7480
		0.744
S4	150pg/ml	0.384	0.3805
		0.377
S5	75pg/ml	0.169	0.1780
		0.187

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