Summary:

 

Purpose

 

Our Quick Step Human Kynurenine (KYN)elisa Kit is to assay KYN levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to KYN. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for KYN is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain KYN and HRP conjugated KYN antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of KYN. You can calculate the concentration of KYN in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2283Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pmol/ml	0.043	0.0405
		0.038
S1	90pmol/ml	1.954	1.9615
		1.969
S2	60pmol/ml	1.318	1.3305
		1.343
S3	30pmol/ml	0.683	0.6910
		0.699
S4	15pmol/ml	0.391	0.3865
		0.382
S5	7.5pmol/ml	0.194	0.1910
		0.188

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