Summary:

 

Purpose

 

Our Quick Step Human Cystathionine Gamma Lyase  Antibody (CSE)ELISA Kit is to assay CSE levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to CSE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for CSE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CSE and HRP conjugated CSE antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CSE. You can calculate the concentration of CSE in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3769Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μmol/L	0.043	0.0405
		0.038
S1	90μmol/L	1.954	1.9615
		1.969
S2	60μmol/L	1.318	1.3305
		1.343
S3	30μmol/L	0.683	0.6910
		0.699
S4	15μmol/L	0.391	0.3865
		0.382
S5	7.5μmol/L	0.194	0.1910
		0.188

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